bifunctional reagents การใช้

• The third category involves cross-linking of enzyme aggregates or crystals, using a bifunctional reagent, to prepare carrier-free macroparticles.
• Traditionally, the recognition of protein-protein interactions was carried out through chemical cross-linking, that involved the use of moderately reactive bifunctional reagent, commonly attached to free amino groups.
• The conventional model for organization of FAS ( see the'head-to-tail'model on the right ) is largely based on the observations that the bifunctional reagent 1, 3-dibromopropanone ( DBP ) is able to crosslink the active site cysteine thiol of the KS domain in one FAS monomer with the phosphopantetheine prosthetic group of the ACP domain in the other monomer.